The effect of oral feeding on gastric emptying, small bowel water content, superior mesenteric artery blood flow, plasma hormone concentrations and blood volume in obese and healthy weight subjects

1st June 2016 - ongoing

Status: In Recruitment

Specialism: Surgical, Peri Operative, Nutrition and Metabolism.

Team: Professor Dileep Lobo.

This nutritional study will investigate the metabolic and physiological changes associated with obese subjects. The results from this may pave the way to a better understanding of the pathogenesis and maintenance of obesity, a condition which can predispose to conditions such as type 2 diabetes and liver cirrhosis.

1. Background

The rate of gastric emptying may have an impact on satiety and has been implied in the pathogenesis of obesity. This may be due to a difference in gastric emptying of food in overweight individuals, or to hormones such as ghrelin, GLP-1, and PYY2.

2. Aims

To establish the different effects of food ingestion on gastric emptying, gallbladder contraction, small bowel water and superior mesenteric artery (SMA) blood flow as measured by serial MRI in the context of changes in plasma gastrointestinal hormone secretion in human subjects who are obese or healthy weight. We hypothesise that nutrient absorption in obese and healthy weight individuals is determined by differences in gastric emptying and mesenteric blood flow which give rise to different insulin, glucose and gut hormone responses.

3. Experimental protocol and methods

24 male subjects aged 18-60 years without coexisting disease: 12 with a BMI of 20-25 and 12 with a BMI 30-35. Following a standard diet for 3 days before the study visit, participants will report at 0900 following an overnight fast. They will undergo an MRI scan to determine baseline values for SMA blood flow, small bowel water content and gastric fluid and gas volumes.Visceral and subcutaneous fat volumes will also be assessed.

The subjects will then be given the test meal based on a pasta, cheese and tomato sauce recipe and consume it within 15 minutes. 20 mL blood samples will be collected prior to the test meal, and then after the meal at 15 and 30 min, and then every 30 min during the 4 h postprandial period and analysed for haemoglobin, glucose, insulin, GLP-1, GLP-2, ghrelin, PYY and serum osmolality. Following the test meal the subjects will be scanned at 30-minute intervals for 4 hours. Satiety questionnaires will be completed on 5 occasions.

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professor-dileep-lobo Professor Dileep Lobo Dileep Lobo is Professor of Gastrointestinal Surgery at the University of Nottingham and Consultant Hepatopancreaticobiliary Surgeon at Queens Medical Centre, Nottingham, UK. He qualified from Bangalore University, India and has trained as a surgeon in…
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